Want to know the structure of a medium sized molecule (~1000Da) and your NOESY is empty – measure EASY-ROESY. A pulse-sequence robust and easy to setup for everybody.
C.M. Thiele, K. Petzold and J. Schleucher“EASY ROESY: reliable cross-peak integration in adiabatic symmetrized ROESY”, Chemistry – A European Journal; 15 (3), pp 585-588, 2009
This sequence allows identification of compounds connected to phosphor, especially in biological mixtures, such as phosphor-lipids.
K. Petzold, A. Olofsson, A. Arnqvist, G. Gröbner and J. Schleucher “Semiconstant-Time P,H-COSY NMR: Analysis of Complex Mixtures of Phospholipids Originating from Helicobacter pylori”, Journal of the American Chemical Society;131 (40), pp 14150–14151, 2
13C and 15N labels are too expensive? Measure you un-labeled RNA dynamics using our SELOPE approach
J. Schlagnitweit, E. Steiner, H. Karlsson and K. Petzold “Efficient detection of structure and dynamics in unlabeled RNAs: the SELOPE approach” Chemistry – A European Journal; 24(23):6067-6070, 2018.
Steiner E., Schlagnitweit J., Lundström P. and Petzold K. “Capturing Excited States in the Fast-Intermediate Exchange Time-Limit in Biological Systems Using 1H NMR Spectroscopy”, Angew Chem Int Edit 2016, 55 (51), 15869–15872, 2016
RNA wetlab with HPLC for RNA purification and solid-phase synthesizer for production