Want to know the structure of a medium sized molecule (~1000Da) and your NOESY is empty – measure EASY-ROESY. A pulse-sequence robust and easy to setup for everybody.
Easy ROESY – Link to PDF HERE
C.M. Thiele, K. Petzold and J. Schleucher“EASY ROESY: reliable cross-peak integration in adiabatic symmetrized ROESY”, Chemistry – A European Journal; 15 (3), pp 585-588, 2009
This sequence allows identification of compounds connected to phosphor, especially in biological mixtures, such as phosphor-lipids (see picture).
P,H-COSY – Link to PDF HERE
K. Petzold, A. Olofsson, A. Arnqvist, G. Gröbner and J. Schleucher “Semiconstant-Time P,H-COSY NMR: Analysis of Complex Mixtures of Phospholipids Originating from Helicobacter pylori”, Journal of the American Chemical Society;131 (40), pp 14150–14151, 2
600 MHz Bruker with 5mm HCNP Cryo-probe, 1.7mm HCN Cryo-probe and Smart probe, cooled sample changer
RNA wetlab with HPLC for RNA sample production